Organometallic photonucleases: synthesis and DNA-cleavage studies of cyclopentadienyl metal-substituted dendrimers designed to increase double-strand scission.
نویسندگان
چکیده
[reaction: see text] A series of metal complex-substituted polyamine dendrimers have been synthesized and examined for their ability to cleave plasmid DNA in a double-stranded manner. While photolysis of the dimetallic spermine derivative and the tetrametallic DAB-Am-4 complex led to double-strand scission, in the larger DAB-Am-8 and DAB-Am-16 systems, DNA aggregation/precipitation was the predominant competing process observed.
منابع مشابه
Photoinduced DNA cleavage by cyclopentadienyl metal complexes conjugated to DNA recognition elements.
[structure: see text]. DNA recognition elements have been attached to CpW(CO)3CH3 and CpW(CO)3Ph, which produce methyl and phenyl radicals that cleave DNA upon photolysis. The inclusion of binding moieties in 3 increases the efficiency but not the selectivity of strand scission over that seen in the simple unfunctionalized complex, while 11 cleaves preferentially at T sites within AT-rich tracts.
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[Reaction: see text]. The photolysis of CpW(CO)3Me has been shown to produce methyl radicals and to cleave DNA in a single-stranded manner, and preliminary evidence implicated a carbon-centered radical in this process. In this work, the mechanism of strand scission in this reaction was determined to occur by hydrogen atom abstraction from the 4'- and 5'-positions of the deoxyribose moiety of th...
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ورودعنوان ژورنال:
- Organic letters
دوره 2 18 شماره
صفحات -
تاریخ انتشار 2000